The p38-MITOGEN-ACTIVATED PROTEIN KINASE Signaling Pathway Is Involved in Leonurus artemisia Extract-Induced Inhibition of the Proliferation of Human Bladder Cancer BFTC-905 Cells via G1/G0 Arrest and Causes Apoptosis In Vitro

Bladder cancer is a urothelial malignancy. starts in the cells lining inside of bladder. The 5-year recurrence rate for bladder ranges from 31% to 78%, and progression approximately 45%. To treat cancer, intravesical drug therapy often used. Leonurus artemisia extract (LaE) was obtained medicinal samples Chinese motherwort Scientific Medicine; L. has various biological effects. This study investigated impact LaE on human (the BFTC-905 cell line) molecular mechanism underlying apoptosis resulting activation signal transduction pathways cells. A counting kit-8 (CCK-8) assay used determine effect growth. migration ability observed using wound healing assay. effects cycle, reactive oxygen species production, were investigated. Western blot analysis detected apoptosis-related mitogen-activated protein kinase signaling pathway-related concentrations. At non-toxic concentrations, inhibited proliferation concentration-dependent manner, half-maximal inhibitory concentration (IC50) 24.08172 µg/µL. impaired arrested cycle G1 G0 phases, increased induced apoptosis. Bax p-ERK concentrations decreased Bcl-2, cleaved caspase-3, p-p38 No differences PARP, C-PARP, vimentin, e-cadherin, p-JNK, or TNF-alpha observed. These results suggest that inhibits Moreover, pathway involved inhibition